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Blue fluorescent protein TagBFP
- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications
Available variants and fusions
Variant | Description | Related vector | Cat.# | Click for image |
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Humanized TagBFP
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TagBFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
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pTagBFP-C
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FP171
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pTagBFP-N
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FP172
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TagBFP-H2B fusion
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Human histone H2B is fused to the TagBFP N-terminus. When expressed in mammalian cells, this fusion provides blue fluorescent labeling of histone H2B in living cells.
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pTagBFP-H2B
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FP176
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References:
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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