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Cyan-to-green photoswitchable fluorescent protein PS-CFP2

- Monomer, succesful perfomance in fusions
- Irreversible photoconversion from a cyan to a green fluorescent form
- High contrast of photoconversion
- High pH stability allowing labeling of acidic organelles
- Recommended for tracking cell, organelle, and protein movement, monitoring the protein turnover and superresolution imaging

PS-CFP2 is an improved mutant of the photoswitchable monomeric fluorescent protein PS-CFP [Chudakov et al., 2004]. PS-CFP2 exhibits faster maturation and a brighter fluorescence both before and after photoswitching than its parental variant.

PS-CFP2 is capable of irreversible photoconversion from cyan to green fluorescent form in response to 405 nm light irradiation. It is recommended for real-time in vivo tracking movement of individual cells, organelles, and protein fractions [Chudakov et al., 2007]. It can also be applied for monitoring of the protein turnover at the single cell level [Zhang et al., 2007] and superresolution imaging by PALM [Shroff et al., 2007]. In addition, PS-CFP2 can be used as a routine cyan fluorescent tag (excitation maximum at 400 nm and emission maximum at 468 nm) at moderate excitation intensities and as a donor in FRET applications [Souslova and Chudakov, 2006].

Related products
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.

PS-CFP2 expression/source vectors

pPS-CFP2-C FP801 Mammalian expression vector encoding humanized PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 C-terminus 20 μg € 400 / 200*
pPS-CFP2-N FP802 Mammalian expression vector encoding humanized PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 N-terminus 20 μg € 400 / 200*

Antibody against PS-CFP2

AB011 Rabbit polyclonal antibody against EGFP, BrUSLEE, SLIM, TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, AceGFP1, Case12 and HyPer 100 μg € 220
AB121 Rabbit polyclonal antibody against TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, Case12, HyPer, EGFP, BrUSLEE and SLIM 100 μg € 220


  • Chudakov DM, Lukyanov S, Lukyanov KA. Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2. Nat Protoc. 2007; 2 (8):2024-32. / pmid: 17703215
  • Chudakov DM, Verkhusha VV, Staroverov DB, Souslova EA, Lukyanov S, Lukyanov KA. Photoswitchable cyan fluorescent protein for protein tracking. Nat Biotechnol. 2004; 22 (11):1435-9. / pmid: 15502815
  • Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych S, Davidson MW, Betzig E. Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes. Proc Natl Acad Sci U S A. 2007; 104 (51):20308-13. / pmid: 18077327
  • Souslova EA, Chudakov DM. Photoswitchable cyan fluorescent protein as a FRET donor. Microsc Res Tech. 2006; 69 (3):207-9. / pmid: 16538627
  • Zhang L, Gurskaya NG, Merzlyak EM, Staroverov DB, Mudrik NN, Samarkina ON, Vinokurov LM, Lukyanov S, Lukyanov KA. Method for real-time monitoring of protein degradation at the single cell level. Biotechniques. 2007; 42 (4):446, 448, 450. / pmid: 17489230
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