Caspase-3 apoptosis sensor Casper3-BG

- Early detection of Caspase-3 activity onset
- High sensitivity
- Direct expression in cells
- No exogenous chemical compounds required
- Recommended for early detection of apoptosis

Main properties

Excitation (thin lines) and emission (thick lines) spectra of TagBFP (blue) and TagGFP2 (green) are shown individually. Spectral overlap is filled with gray. Download Casper3-BG spectra (xls)

CHARACTERISTIC   FRET donor TagBFP     Fluorescence color blue     Excitation maximum, nm 402     Emission maximum, nm 457     Brightness, % of EGFP 99     pKa 2.7 FRET acceptor TagGFP2     Fluorescence color green     Excitation maximum, nm 483     Emission maximum, nm 506     Brightness, % of EGFP 105     pKa 5.0 Calculated Förster distance R0 5.25 FRET efficiency E 0.57 Specificity caspase-3 activity Response elimination of FRET Polypeptide length, aa 490 Molecular weight, kDa 55