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Vanlangenakker N, Bertrand MJ, Bogaert P, Vandenabeele P, Vanden Berghe T.
TNF-induced necroptosis in L929 cells is tightly regulated by multiple TNFR1 complex I and II members.
Cell Death Dis. 2011 Nov 17;2:e230 doi: 10.1038/cddis.2011.111
pmid: 22089168
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Cell death typing by monitoring caspase 3 activity.
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Constructs used: pCasper3-BG vector.
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Expression system: L929sAhFas murine fibrosarcoma cells, transient expression.
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Detection system: Simultaneous analysis of cell death and caspase-3 activity in L929pCasper3-BG cells was performed by flow cytometry using a triple-laser LSR-II (405, 488 and 635 nm).
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