Calcium ion sensor Case12

- High dynamic range detection of intracellular Ca²⁺ level changes
- High selectivity and sensitivity, relatively high pH stability
- Fast maturation, high brightness of fluorescent response
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required
- Recommended for monitoring changes in Ca²⁺ concentration inside living cells

Main properties

Case12 normalized excitation (blue) and emission (green) spectra without Ca2+ (thin line) and in the presence of 1 mM of Ca2+ (thick line). Download Case12 spectra (xls)	CHARACTERISTIC   Emission maximum, nm 516 Excitation maximum, nm 491 Fluorescence color green Polypeptide length, aa 415 Molecular weight, kDa 46.4 Specificity Ca2+ Kd for Ca2+ 1 μM pKa 7.2 Structure monomer Aggregation no Maturation rate at 37°C fast

	Ca2+ titration curves. The apparent Kd for calcium ions binding was found to be 1 μM, which lies within the physiological range of calcium ions concentrations. Image from Souslova et al., 2007.