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    Case12

Calcium ion sensor Case12

- High dynamic range detection of intracellular Ca2+ level changes
- High selectivity and sensitivity, relatively high pH stability
- Fast maturation, high brightness of fluorescent response
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required
- Recommended for monitoring changes in Ca2+ concentration inside living cells

Main properties

Case12 spectra

Case12 normalized excitation (blue) and emission (green) spectra without Ca2+ (thin line) and in the presence of 1 mM of Ca2+ (thick line).

Download Case12 spectra (xls)

CHARACTERISTIC

 

Emission maximum, nm516
Excitation maximum, nm491
Fluorescence colorgreen
Polypeptide length, aa415
Molecular weight, kDa46.4
SpecificityCa2+
Kd for Ca2+1 μM
pKa7.2
Structuremonomer
Aggregationno
Maturation rate at 37°Cfast

Ca2+ titration curves.

The apparent Kd for calcium ions binding was found to be 1 μM, which lies within the physiological range of calcium ions concentrations. Image from Souslova et al., 2007.

References:

  • Souslova EA, Belousov VV, Lock J, Stromblad S, Kasparov S, Bolshakov AP, Pinelis VG, Labas YA, Lukyanov S, Mayr LM, Chudakov DM. Single fluorescent protein-based Ca2+ sensors with increased dynamic range. BMC Biotechnol. 2007; 7 :37. / pmid: 17603870
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