mKate2

Far-red fluorescent protein mKate2

- Super bright far-red fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability and photostability, low cytotoxicity
- Proven suitability to generate stably transfected cell lines
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling, multicolor applications and whole body imaging

Main properties

mKate2 spectra

mKate2 normalized excitation (thin line) and emission (thick line) spectra.

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Download mKate2 spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
** Time to bleach 50% of fluorescent signal brightness.
Extinction coefficient and quantum yield were measured at the physiological pH=7.5.
Molecular weight, kDa26
Polypeptide length, aa232
Fluorescence colorfar-red
Excitation maximum, nm588
Emission maximum, nm633
Quantum yield0.40
Extinction coefficient, M-1cm-162 500
Brightness*25.0
Brightness, % of EGFP74
pKa5.4
Structuremonomer
Aggregationno
Maturation half-time, min<20
Photostability, widefield**69
Photostability, confocal**390
Cell toxicitynot observed
Main advantagesbright monomeric far-red fluorescent protein, high pH- and photostability

Spectral characteristics of mKate2 in comparison with selected fluorescent proteins.

(A) Emission spectra of far-red monomeric fluorescent proteins given proportionally to their calculated brightness. Scaling was applied to the area of the peak. Favorable "optical window" is shaded with gray. (B) Normalized photobleaching curves for far-red monomeric fluorescent proteins, laser scanning confocal microscopy. (C) Normalized photobleaching curves, widefield fluorescence microscopy under metal halide illumination. Brown line – mKate2, blue line – mRaspberry, dark-blue line – mPlum.

pH stability of mKate2 (brown line) and TagFP635 (red line) fluorescence.

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