mKate2 normalized excitation (thin line) and emission (thick line) spectra.
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|* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.|
** Time to bleach 50% of fluorescent signal brightness.
Extinction coefficient and quantum yield were measured at the physiological pH=7.5.
|Molecular weight, kDa||26|
|Polypeptide length, aa||232|
|Excitation maximum, nm||588|
|Emission maximum, nm||633|
|Extinction coefficient, M-1cm-1||62 500|
|Brightness, % of EGFP||74|
|Maturation half-time, min||<20|
|Cell toxicity||not observed|
|Main advantages||bright monomeric far-red fluorescent protein, high pH- and photostability|
pH stability of mKate2 (brown line) and TagFP635 (red line) fluorescence.