mKate2

Far-red fluorescent protein mKate2

- Super bright far-red fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability and photostability, low cytotoxicity
- Proven suitability to generate stably transfected cell lines
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling, multicolor applications and whole body imaging


Available variants and fusions
VariantDescriptionRelated vectorCat.#Click for image
Humanized mKate2 mKate2 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. pmKate2-C FP181
pmKate2-N FP182
mKate2-actin fusion Human β-actin is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of β-actin in living cells. pmKate2-actin FP184
mKate2-tubulin fusion Human α-tubulin is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of α-tubulin in living cells. pmKate2-tubulin FP185
mKate2-f-mem fusion 20 amino acid farnesylation signal from c-Ha-Ras is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of plasma membrane. pmKate2-f-mem FP186
mKate2-mito fusion A mitochondrial targeting sequence (MTS) is fused to the mKate2 N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides far-red fluorescent labeling of mitochondria. pmKate2-mito FP187
mKate2-laminB1 fusion Human lamin B1 is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of lamin B1 in living cells. pmKate2-laminB1 FP310
mKate2-H2B fusion Human histone H2B is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of histone H2B in living cells. pmKate2-H2B FP311
mKate2-lyso fusion Rat Lysosomal Associated Membrane Protein 1 (LAMP-1) is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of lysosomes. pmKate2-lyso FP312
mKate2-peroxi fusion Peroxisomal targeting signal [Gould et al., 1989] encoding tripeptide SKL is fused to the 3' end of mKate2 sequence. This tripeptide targets the fusion protein to the matrix of peroxisomes. pmKate2-peroxi FP313
mKate2-endo fusion Human RhoB GTPase is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of vesicles of the endocytic pathway. pmKate2-endo FP314
mKate2-zyxin fusion Human zyxin is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of zyxin in living cells. pmKate2-zyxin FP315
mKate2-EB3 fusion Human microtubule-associated EB3 protein is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of EB3 protein in living cells. pmKate2-EB3 FP316
mKate2-actinin fusion Human α-actinin is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of α-actinin in living cells. pmKate2-actinin FP317
mKate2-vimentin fusion Human vimentin is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of vimentin in living cells. pmKate2-vimentin FP318
mKate2-keratin fusion Human cytokeratin-18 is fused to themKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of cytokeratin-18 in living cells. pmKate2-keratin FP319
mKate2-profilin fusion Mouse profilin is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of profilin in living cells. pmKate2-profilin FP320
mKate2-annexin fusion Human annexin is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of annexin in living cells. pmKate2-annexin FP321
mKate2-clathrin fusion Human clathrin LCB is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of clathrin LCB in living cells. pmKate2-clathrin FP322
mKate2-paxillin fusion Chicken paxillin is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of paxillin in living cells. pmKate2-paxillin FP323
mKate2-ER fusion Signal sequence of calreticulin is fused to the mKate2 N-terminus and the endoplasmic reticulum retention sequence KDEL is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of the lumen of the endoplasmic reticulum. pmKate2-ER FP324


References:

  • Fliegel L, Burns K, MacLennan DH, Reithmeier RA, Michalak M. Molecular cloning of the high affinity calcium-binding protein (calreticulin) of skeletal muscle sarcoplasmic reticulum. J Biol Chem. 1989; 264 (36):21522-8. / pmid: 2600080
  • Gould SJ, Keller GA, Hosken N, Wilkinson J, Subramani S. A conserved tripeptide sorts proteins to peroxisomes. J Cell Biol. 1989; 108 (5):1657-64. / pmid: 2654139
  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
  • Munro S, Pelham HR. A C-terminal signal prevents secretion of luminal ER proteins. Cell. 1987; 48 (5):899-907. / pmid: 3545499
  • Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
  • Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA. A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues. J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
  • Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Far-red fluorescent tags for protein imaging in living tissues. Biochem J. 2009; 418 (3):567-74. doi: 10.1042/BJ20081949 / pmid: 19143658
Copyright 2002-2016 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com