TurboRFP

Red (orange) fluorescent protein TurboRFP

- Super bright red (orange) fluorescence
- Fast maturation, high pH-stability
- Recommended for gene expression analysis and cell and organelle labeling


Available variants and fusions
VariantDescriptionRelated vectorCat.#Click for image
Humanized TurboRFP TurboRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. pTurboRFP-C FP231
pTurboRFP-N FP232
pTurboRFP-B FP233
pTurboRFP-PRL FP235
TurboRFP-mito fusion A mitochondrial targeting sequence (MTS) is fused to the TurboRFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of mitochondria. pTurboRFP-mito FP237


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
  • Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
  • Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA. A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues. J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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