pTurboRFP-B

pTurboRFP-B vector

cat.# FP233

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pTurboRFP-BFP23320 μg€ 320 / 160*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Vector typebacterial expression vector
ReporterTurboRFP
Reporter codon usagemammalian
Promoter for TurboRFPT5 promoter/lac operator
Host cellsprokaryotic
Selectionampicillin
ReplicationColE1 ori
Use Source of the TurboRFP coding sequence; TurboRFP expression in bacterial cells
5'-Region
TurboRFP
BamH I
RBS ATG. AGA.GGA.TCG. GGA.TCC. ATG.AGC.GAG...
3'-Region
Stop
Hind III
...GGG.CAC.AGA. TG A. AGC.TT

Vector description

pTurboRFP-B is a prokaryotic expression vector encoding red (orange) fluorescent protein TurboRFP (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of TurboRFP coding sequence. Flanking restriction sites are convenient for excision of TurboRFP sequence and its further insertion into other expression vectors of choice. Alternatively, TurboRFP coding sequence can be amplified by PCR.

Note: The plasmid DNA was isolated from dam+-methylated E. coli. Therefore some restriction sites are blocked by methylation. If you wish to digest the vector using such sites you will need to transform the vector into a dam- host and make fresh DNA.

The vector can be also used for TurboRFP expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.


Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
TurboRFP coding sequence: 132-827
Lambda t0 transcriptional termination region: 848-942
rrnB T1 transcriptional termination region: 1704-1802
ColE1 origin of replication: 2278
beta-lactamase coding sequence: 3896-3036


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248

Notice to Purchaser:

TurboRFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 8,138,320; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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