NirFP

Near-infrared fluorescent protein NirFP

- Near-infrared fluorescence with emission maximum at 670 nm
- Extremely high photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for multicolor applications

Main properties

NirFP spectra

NirFP normalized excitation (thin line) and emission (thick line) spectra.

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Download NirFP spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
** Brightness in infrared is a product of extinction coefficient at 635 nm, quantum yield and emission fraction between 700 nm and 900 nm, divided by 1000.
Molecular weight, kDa26
Polypeptide length, aa234
Fluorescence colornear-infrared
Excitation maximum, nm605
Emission maximum, nm670
Quantum yield0.06
Extinction coefficient, M-1cm-170 000
Brightness*4.2
Brightness, % of EGFP13
Extinction coefficient, M-1cm-1 at 635 nm15 700
Quantum yield in infrared (700-900 nm)0.03
Brightness in infrared**0.47
pKa4.5
Structuredimer
Aggregationno
Photostabilitysuper high
Cell toxicitynot observed
Main advantagesmost red-shifted fluorescent protein available; high pH-stability, extremely high photostability
Possible limitationsdimer, limited applicability for fusions generation; low brightness
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