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pmKate2-mito vector

cat.# FP187

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pmKate2-mitoFP18720 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for mKate2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use far-red fluorescent labeling of mitochondria

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of mKate2 coding sequence (ATG): 705-707
Stop codon: 1401-1403
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1556-1561 & 1585-1590
mRNA 3' ends: 1594 & 1606
f1 single-strand DNA origin: 1653-2108
Bacterial promoter for expression of Kanr gene
-35 region: 2170-2175
-10 region: 2193-2198
Transcription start point: 2205
SV40 origin of replication: 2449-2584
SV40 early promoter
Enhancer (72-bp tandem repeats): 2282-2353 & 2354-2425
21-bp repeats: 2429-2449, 2450-2470 & 2472-2492
Early promoter element: 2505-2511
Major transcription start points: 2501, 2539, 2545 & 2550
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2633-2635
Stop codon: 3425-3427
G->A mutation to remove Pst I site: 2815
C->A (Arg to Ser) mutation to remove BssH II site: 3161
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3663-3668 & 3676-3681
pUC plasmid replication origin: 4012-4655

Notice to Purchaser:

mKate2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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