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RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs:

    mKate2

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References citing mKate2 or TagFP635:
  • Mamontova AV, Solovyev ID, Savitsky AP, Shakhov AM, Lukyanov KA, Bogdanov AM.
    Bright GFP with subnanosecond fluorescence lifetime.
    Sci Rep. 2018 Sep 5;8(1):13224 doi: 10.1038/s41598-018-31687-w.
    pmid: 30185895
     
  • de Jong NW, van der Horst T, van Strijp JA, Nijland R.
    Fluorescent reporters for markerless genomic integration in Staphylococcus aureus.
    Sci Rep. 2017 Mar 7;7:43889 doi: 10.1038/srep43889
    pmid: 28266573
     
  • Rouxel RN, Mérour E, Biacchesi S, Brémont M.
    Efficient Co-Replication of Defective Novirhabdovirus.
    Viruses. 2016 Mar 4;8(3):pii: E69 doi: 10.3390/v8030069
    pmid: 26959049
     
  • Kong Y, Yang D, Cirillo SL, Li S, Akin A, Francis KP, Maloney T, Cirillo JD.
    Application of Fluorescent Protein Expressing Strains to Evaluation of Anti-Tuberculosis Therapeutic Efficacy In Vitro and In Vivo.
    PLoS One. 2016 Mar 2;11(3):e0149972 doi: 10.1371/journal.pone.0149972
    pmid: 26934495
     
  • Beilharz K, van Raaphorst R, Kjos M, Veening JW.
    Red fluorescent proteins for gene expression and protein localization studies in Streptococcus pneumoniae and efficient transformation with Gibson assembled DNA.
    Appl Environ Microbiol. 2015 Aug 7;pii: AEM.02033-15. [Epub ahead of print]
    pmid: 26253684
     
  • Detert Oude Weme RG, Kovács ÁT, de Jong SJ, Veening JW, Siebring J, Kuipers OP.
    Single cell FRET analysis for the identification of optimal FRET-pairs in Bacillus subtilis using a prototype MEM-FLIM system.
    PLoS One. 2015 Apr 17;10(4):e0123239 doi: 10.1371/journal.pone.0123239
    pmid: 25886351
     
  • Zhang X, Wang D, Zhao S, Shen Z.
    A double built-in containment strategy for production of recombinant proteins in transgenic rice.
    PLoS One. 2014 Dec 22;9(12):e115459 doi: 10.1371/journal.pone.0115459
    pmid: 25531447
     
  • Ronen M, Shalaby S, Horwitz BA.
    Role of the transcription factor ChAP1 in cytoplasmic redox homeostasis: imaging with a genetically encoded sensor in the maize pathogen Cochliobolus heterostrophus.
    Mol Plant Pathol. 2013 Oct;14(8):786-90 doi: 10.1111/mpp.12047
    pmid: 23745603
  1. Constructs used: HyPer was expressed under the control of a fungal promoter, PTrpC, from pUCATPH.
  2. Expression system: Cochliobolus heterostrophus.
  3. Detection system: Zeiss LSM 700 confocal microscope.
  • Rosenfeld GE, Mercer EJ, Mason CE, Evans T.
    Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis.
    Dev Biol. 2013 Sep 15;381(2):389-400 doi: 10.1016/j.ydbio.2013.06.025
    pmid: 23850773
  1. A study of heart morphogenesis in transgenic zebrafish expressing mKate-Caax. Anti-tRFP antibody was used for immunohistochemical staining of intact fish embryos.
  2. Constructs used: mKate-Caax fusion.
  3. Expression system: Transgenic zebrafish line.
  • Eijlander RT, Kuipers OP.
    Live-cell imaging tool optimization to study gene expression levels and dynamics in single cells of Bacillus cereus.
    Appl Environ Microbiol. 2013 Sep;79(18):5643-51 doi: 10.1128/AEM.01347-13
    pmid: 23851094
     
  • Xiang Z, Ren H, Hu YS, Coin I, Wei J, Cang H, Wang L.
    Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity.
    Nat Methods. 2013 Sep;10(9):885-8 doi: 10.1038/nmeth.2595
    pmid: 23913257
     
  • Gaytán P, Roldán-Salgado A.
    Elimination of redundant and stop codons during the chemical synthesis of degenerate oligonucleotides. Combinatorial testing on the chromophore region of the red fluorescent protein mKate.
    ACS Synth Biol. 2013 Aug 16;2(8):453-62 doi: 10.1021/sb3001326
    pmid: 23654278
  1. Random mutagenesis of emKate (mKateS158A); original mKate was used for comparison of spectral characteristics with new FP variants.
  • Guess MG, Barthel KK, Pugach EK, Leinwand LA.
    Measuring microRNA reporter activity in skeletal muscle using hydrodynamic limb vein injection of plasmid DNA combined with in vivo imaging.
    Skelet Muscle. 2013 Aug 1;3(1):19 doi: 10.1186/2044-5040-3-19
    pmid: 23915674
     
  • Loots GG, Bergmann A, Hum NR, Oldenburg CE, Wills AE, Hu N, Ovcharenko I, Harland RM.
    Interrogating transcriptional regulatory sequences in Tol2-mediated Xenopus transgenics.
    PLoS One. 2013 Jul 16;8(7):e68548 doi: 10.1371/journal.pone.0068548
    pmid: 23874664
     
  • Brenner MH, Cai D, Swanson JA, Ogilvie JP.
    Two-photon imaging of multiple fluorescent proteins by phase-shaping and linear unmixing with a single broadband laser.
    Opt Express. 2013 Jul 15;21(14):17256-64 doi: 10.1364/OE.21.017256
    pmid: 23938572
     
  • Lee S, Lim WA, Thorn KS.
    Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae.
    PLoS One. 2013 Jul 2;8(7):e67902 doi: 10.1371/journal.pone.0067902
    pmid: 23844123
     
  • Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR.
    Improved tools for the Brainbow toolbox.
    Nat Methods. 2013 Jun;10(6):540-7
    pmid: 23866336
  1. mKate2 was selected for the Brainbow 3.0 constructs. PhiYFP, TagBFP, mKate2, TurboFP650 were tested for tendency to aggregate, photostability in vivo and stability after formaldehyde fixation, and also for minimal spectral overlapping and minimal sequence homology. Anti-PhiYFP antibody was used for PhiYFP detection.
  • Vijftigschild LA, van der Ent CK, Beekman JM.
    A novel fluorescent sensor for measurement of CFTR function by flow cytometry.
    Cytometry A. 2013 Jun;83(6):576-84 doi: 10.1002/cyto.a.22275
    pmid: 23463616
  1. A new biosensor for CFTR (Cystic fibrosis transmembrane conductance regulator) activity was developed. mKate2 was used in combination with YFP for ratiometric measurement of halide ions.
  • Krylova I, Kumar RR, Kofoed EM, Schaufele F.
    A versatile, bar-coded nuclear marker/reporter for live cell fluorescent and multiplexed high content imaging.
    PLoS One. 2013 May 14;8(5):e63286 doi: 10.1371/journal.pone.0063286
    pmid: 23691010
     
  • Klimenko OV, Shtilman MI.
    Transfection of Kasumi-1 cells with a new type of polymer carriers loaded with miR-155 and antago-miR-155.
    Cancer Gene Ther. 2013 Apr;20(4):237-41 doi: 10.1038/cgt.2013.11
    pmid: 23538486
     
  • Kitaguchi T, Oya M, Wada Y, Tsuboi T, Miyawaki A.
    Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells.
    Biochem J. 2013 Mar 1;450(2):365-73 doi: 10.1042/BJ20121022
    pmid: 23282092
     
  • Bonekamp NA, Islinger M, Lázaro MG, Schrader M.
    Cytochemical detection of peroxisomes and mitochondria.
    Methods Mol Biol. 2013;931:467-82 doi: 10.1007/978-1-62703-056-4_24
    pmid: 23027018
  1. Protocols for dual peroxisomes and mitochondria staining.
  • Hotard AL, Shaikh FY, Lee S, Yan D, Teng MN, Plemper RK, Crowe JE Jr, Moore ML.
    A stabilized respiratory syncytial virus reverse genetics system amenable to recombination-mediated mutagenesis.
    Virology. 2012 Dec 5;434(1):129-36 doi: 10.1016/j.virol.2012.09.022
    pmid: 23062737
     
  • Salomonnson E, Mihalko LA, Verkhusha VV, Luker KE, Luker GD.
    Cell-based and in vivo spectral analysis of fluorescent proteins for multiphoton microscopy.
    J Biomed Opt. 2012 Sep 1;17(9):96001 doi: 10.1117/1.JBO.17.9.096001
    pmid: 22975677
     
  • Telford WG, Hawley T, Subach F, Verkhusha V, Hawley RG.
    Flow cytometry of fluorescent proteins.
    Methods. 2012 Jul;57(3):318-30 doi: 10.1016/j.ymeth.2012.01.003
    pmid: 22293036
     
  • Anderson DD, Woeller CF, Chiang EP, Shane B, Stover PJ.
    Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis.
    J Biol Chem. 2012 Mar 2;287(10):7051-62 doi: 10.1074/jbc.M111.333120
    pmid: 22235121
     
  • Numata T, Sato K, Christmann J, Marx R, Mori Y, Okada Y, Wehner F.
    The ΔC splice-variant of TRPM2 is the hypertonicity-induced cation channel in HeLa cells, and the ecto-enzyme CD38 mediates its activation.
    J Physiol. 2012 Mar 1;590(Pt 5):1121-38 doi: 10.1113/jphysiol.2011.220947
    pmid: 22219339
     
  • Miranda JG, Weaver AL, Qin Y, Park JG, Stoddard CI, Lin MZ, Palmer AE.
    New alternately colored FRET sensors for simultaneous monitoring of Zn(2+) in multiple cellular locations.
    PLoS One. 2012;7(11):e49371 doi: 10.1371/journal.pone.0049371
    pmid: 23173058
     
  • Nadell CD, Bassler BL.
    A fitness trade-off between local competition and dispersal in Vibrio cholerae biofilms.
    Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14181-5 doi: 10.1073/pnas.1111147108
    pmid: 21825170
     
  • Snaith HA, Thompson J, Yates JR 3rd, Sawin KE.
    Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
    J Cell Sci. 2011 Jul 1;124(Pt 13):2187-99 doi: 10.1242/jcs.084038
    pmid: 21652630
     
  • Verreault M, Strutt D, Masin D, Fink D, Gill R, Bally MB.
    Development of glioblastoma cell lines expressing red fluorescence for non-invasive live imaging of intracranial tumors.
    Anticancer Res. 2011 Jun;31(6):2161-71
    pmid: 21737636
     
  • Watson DS, Platt VM, Cao L, Venditto VJ, Szoka FC Jr.
    Antibody response to polyhistidine-tagged peptide and protein antigens attached to liposomes via lipid-linked nitrilotriacetic acid in mice.
    Clin Vaccine Immunol. 2011 Feb;18(2):289-97 doi: 10.1128/CVI.00425-10
    pmid: 21159923
     
  • Mutoh H, Perron A, Akemann W, Iwamoto Y, Knöpfel T.
    Optogenetic monitoring of membrane potentials.
    Exp Physiol. 2011 Jan;96(1):13-8 doi: 10.1113/expphysiol.2010.053942
    pmid: 20851856
     
  • Wang Q, Byrnes LJ, Shui B, Röhrig UF, Singh A, Chudakov DM, Lukyanov S, Zipfel WR, Kotlikoff MI, Sondermann H.
    Molecular mechanism of a green-shifted, pH-dependent red fluorescent protein mKate variant.
    PLoS One. 2011;6(8):e23513 doi: 10.1371/journal.pone.0023513
    pmid: 21887263
     
  • Piatkevich KD, Hulit J, Subach OM, Wu B, Abdulla A, Segall JE, Verkhusha VV.
    Monomeric red fluorescent proteins with a large Stokes shift.
    Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5369-74 doi: 10.1073/pnas.0914365107
    pmid: 20212155
     
  • Pletnev S, Gurskaya NG, Pletneva NV, Lukyanov KA, Chudakov DM, Martynov VI, Popov VO, Kovalchuk MV, Wlodawer A, Dauter Z, Pletnev V.
    Structural basis for phototoxicity of the genetically encoded photosensitizer KillerRed.
    J Biol Chem. 2009 Nov 13;284(46):32028-39 doi: 10.1074/jbc.M109.054973
    pmid: 19737938
     
  • Chu J, Zhang Z, Zheng Y, Yang J, Qin L, Lu J, Huang ZL, Zeng S, Luo Q.
    A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions.
    Biosens Bioelectron. 2009 Sep 15;25(1):234-9 doi: 10.1016/j.bios.2009.06.008
    pmid: 19596565
     
  • Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien RY.
    Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome.
    Science. 2009 May 8;324(5928):804-7 doi: 10.1126/science.1168683
    pmid: 19423828
     
  • Nowotschin S, Eakin GS, Hadjantonakis AK.
    Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives.
    Trends Biotechnol. 2009 May;27(5):266-76 doi: 10.1016/j.tibtech.2009.02.006
    pmid: 19339068
     
  • Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM.
    Far-red fluorescent tags for protein imaging in living tissues.
    Biochem J. 2009 Mar 15;418(3):567-74 doi: 10.1042/BJ20081949
    pmid: 19143658
     
  • Gautam SG, Perron A, Mutoh H, Knopfel T.
    Exploration of fluorescent protein voltage probes based on circularly permuted fluorescent proteins.
    Front Neuroengineering. 2009;2:14 doi: 10.3389/neuro.16.014.2009
    pmid: 19862342
     
  • Pletnev S, Shcherbo D, Chudakov DM, Pletneva N, Merzlyak EM, Wlodawer A, Dauter Z, Pletnev V.
    A crystallographic study of bright far-red fluorescent protein mKate reveals pH-induced cis-trans isomerization of the chromophore.
    J Biol Chem. 2008 Oct 24;283(43):28980-7 doi: 10.1074/jbc.M800599200
    pmid: 18682399
     
  • Ouyang M, Sun J, Chien S, Wang Y.
    Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors.
    Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14353-8 doi: 10.1073/pnas.0807537105
    pmid: 18799748
     
  • Manna D, Bhardwaj N, Vora MS, Stahelin RV, Lu H, Cho W.
    Differential roles of phosphatidylserine, PtdIns(4,5)P2, and PtdIns(3,4,5)P3 in plasma membrane targeting of C2 domains. Molecular dynamics simulation, membrane binding, and cell translocation studies of the PKCalpha C2 domain.
    J Biol Chem. 2008 Sep 19;283(38):26047-58 doi: 10.1074/jbc.M802617200
    pmid: 18621733
     
  • Held MA, Boulaflous A, Brandizzi F.
    Advances in fluorescent protein-based imaging for the analysis of plant endomembranes.
    Plant Physiol. 2008 Aug;147(4):1469-81 doi: 10.1104/pp.108.120147
    pmid: 18678739
     
  • Stepanenko OV, Verkhusha VV, Kuznetsova IM, Uversky VN, Turoverov KK.
    Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes.
    Curr Protein Pept Sci. 2008 Aug;9(4):338-69 http://asb.aecom.yu.edu
    pmid: 18691124
     
  • Martynova NY, Eroshkin FM, Ermolina LV, Ermakova GV, Korotaeva AL, Smurova KM, Gyoeva FK, Zaraisky AG.
    The LIM-domain protein Zyxin binds the homeodomain factor Xanf1/Hesx1 and modulates its activity in the anterior neural plate of Xenopus laevis embryo.
    Dev Dyn. 2008 Mar;237(3):736-49 doi: 10.1002/dvdy.21471
    pmid: 18297730
     
  • Shaner NC, Patterson GH, Davidson MW.
    Advances in fluorescent protein technology.
    J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60
    pmid: 18057027
     
  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM.
    Bright far-red fluorescent protein for whole-body imaging.
    Nat Methods. 2007 Sep;4(9):741-6
    pmid: 17721542
     

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