pmKate2-lyso vector

cat.# FP312

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pmKate2-lyso FP312 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter mKate2 Reporter codon usage mammalian Promoter for mKate2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use far-red fluorescent labeling of lysosomes

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-LAMP1 fusion
Start codon (ATG): 626-628
Lysosomal Associated Membrane Protein 1 (LAMP1) sequence: 626-1846
Start of mKate2 coding sequence (ATG): 1907-1909
Stop codon: 2603-2605
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2758-2763 & 2787-2792
mRNA 3' ends: 2796 & 2808
f1 single-strand DNA origin: 2855-3310
Bacterial promoter for expression of Kan^r gene
-35 region: 3372-3377
-10 region: 3395-3400
Transcription start point: 3407
SV40 origin of replication: 3651-3786
SV40 early promoter
Enhancer (72-bp tandem repeats): 3484-3555 & 3556-3627
21-bp repeats: 3631-3651, 3652-3672 & 3674-3694
Early promoter element: 3707-3713
Major transcription start points: 3703, 3741, 3747 & 3752
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3835-3837
Stop codon: 4627-4629
G->A mutation to remove Pst I site: 4017
C->A (Arg to Ser) mutation to remove BssH II site: 4363
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4865-4870 & 4878-4883
pUC plasmid replication origin: 5214-5857