pmKate2-f-mem vector

cat.# FP186

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pmKate2-f-mem FP186 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter mKate2 Reporter codon usage mammalian Promoter for mKate2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use far-red fluorescent labeling of plasma membrane

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
mKate2
Start codon (ATG): 613-615
Last amino acid in mKate2: 1312-1314
Farnesylation signal: 1330-1389
Stop codon: 1390-1392
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1584-1589 & 1613-1618
mRNA 3' ends: 1622 & 1634
f1 single-strand DNA origin: 1681-2136
Bacterial promoter for expression of Kan^r gene
-35 region: 2198-2203
-10 region: 2221-2226
Transcription start point: 2233
SV40 origin of replication: 2477-2612
SV40 early promoter
Enhancer (72-bp tandem repeats): 2310-2381 & 2382-2453
21-bp repeats: 2457-2477, 2478-2498 & 2500-2520
Early promoter element: 2533-2539
Major transcription start points: 2529, 2567, 2573 & 2578
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2661-2663
Stop codon: 3453-3455
G->A mutation to remove Pst I site: 2843
C->A (Arg to Ser) mutation to remove BssH II site: 3189
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3691-3696 & 3704-3709
pUC plasmid replication origin: 4040-4683