pmKate2-clathrin vector

cat.# FP322

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pmKate2-clathrin FP322 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter mKate2 Reporter codon usage mammalian Promoter for mKate2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use far-red fluorescent labeling of clathrin LCB

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
mKate2-clathrin fusion: 613-1992
mKate2: 613-1314
Start codon (ATG): 613-615
Last amino acid in mKate2: 1312-1314
Clathrin LCB: 1360-1992
Stop codon: 1993-1995
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2156-2161 & 2185-2190
mRNA 3' ends: 2194 & 2206
f1 single-strand DNA origin: 2253-2708
Bacterial promoter for expression of Kan^r gene
-35 region: 2770-2775
-10 region: 2793-2798
Transcription start point: 2805
SV40 origin of replication: 3049-3184
SV40 early promoter
Enhancer (72-bp tandem repeats): 2882-2953 & 2954-3025
21-bp repeats: 3029-3049, 3050-3070 & 3072-3092
Early promoter element: 3105-3111
Major transcription start points: 3101, 3139, 3145 & 3150
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3233-3235
Stop codon: 4025-4027
G->A mutation to remove Pst I site: 3415
C->A (Arg to Ser) mutation to remove BssH II site: 3761
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4263-4268 & 4276-4281
pUC plasmid replication origin: 4612-5255