Eng | Ru

    pmKate2-ER

pmKate2-ER vector

cat.# FP324

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


Download
vector information:
ProductCat.#SizePrice
pmKate2-ERFP32420 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
ReportermKate2
Reporter codon usagemammalian
Promoter for mKate2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use far-red fluorescent labeling of the lumen of the endoplasmic reticulum

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-ER fusion: 597-1391
Start codon (ATG): 597-599
Calreticulin signal sequence: 597-647
mKate2: 663-1358
ER retention sequence (KDEL): 1380-1391
Stop codon: 1392-1394
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1593-1598 & 1622-1627
mRNA 3' ends: 1631 & 1643
f1 single-strand DNA origin: 1690-2145
Bacterial promoter for expression of Kanr gene
-35 region: 2207-2212
-10 region: 2230-2235
Transcription start point: 2242
SV40 origin of replication: 2486-2621
SV40 early promoter
Enhancer (72-bp tandem repeats): 2319-2390 & 2391-2462
21-bp repeats: 2466-2486, 2487-2507 & 2509-2529
Early promoter element: 2542-2548
Major transcription start points: 2538, 2576, 2582 & 2587
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2670-2672
Stop codon: 3462-3464
G->A mutation to remove Pst I site: 2852
C->A (Arg to Ser) mutation to remove BssH II site: 3198
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3700-3705 & 3713-3718
pUC plasmid replication origin: 4049-4692


Notice to Purchaser:

mKate2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

Copyright 2002-2020 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com