pmKate2-C vector

cat.# FP181

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pmKate2-C FP181 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter mKate2 Reporter codon usage mammalian Promoter for mKate2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use mKate2 expression in mammalian cells; generation of fusions to the mKate2 C-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1399-1401
Last amino acid in mKate2: 1306-1308
MCS: 1321-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1541-1546 & 1570-1575
mRNA 3' ends: 1579 & 1591
f1 single-strand DNA origin: 1638-2093
Bacterial promoter for expression of Kan^r gene
-35 region: 2155-2160
-10 region: 2178-2183
Transcription start point: 2190
SV40 origin of replication: 2434-2569
SV40 early promoter
Enhancer (72-bp tandem repeats): 2267-2338 & 2339-2410
21-bp repeats: 2414-2434, 2435-2455 & 2457-2477
Early promoter element: 2490-2496
Major transcription start points: 2486, 2524, 2530 & 2535
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2618-2620
Stop codon: 3410-3412
G->A mutation to remove Pst I site: 2800
C->A (Arg to Ser) mutation to remove BssH II site: 3146
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3648-3653 & 3661-3666
pUC plasmid replication origin: 3997-4640