pTagGFP2-tubulin vector

cat.# FP195

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagGFP2-tubulin FP195 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagGFP2 Reporter codon usage mammalian Promoter for TagGFP2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use green fluorescent labeling of α-tubulin filaments

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagGFP2
Kozak consensus translation initiation site: 600-610
Start codon (ATG): 607-609
Stop codon: 2692-2694
Last amino acid in TagGFP2: 1318-1320
Tubulin: 1339-2694
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2855-2860 & 2884-2889
mRNA 3' ends: 2893 & 2905
f1 single-strand DNA origin: 2952-3407
Bacterial promoter for expression of Kan^r gene
-35 region: 3469-3474
-10 region: 3492-3497
Transcription start point: 3504
SV40 origin of replication: 3748-3883
SV40 early promoter
Enhancer (72-bp tandem repeats): 3581-3652 & 3653-3724
21-bp repeats: 3728-3748, 3749-3769 & 3771-3791
Early promoter element: 3804-3810
Major transcription start points: 3800, 3838, 3844 & 3849
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3932-3934
Stop codon: 4724-4726
G->A mutation to remove Pst I site: 4114
C->A (Arg to Ser) mutation to remove BssH II site: 4460
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4962-4967 & 4975-4980
pUC plasmid replication origin: 5311-5954