pTagGFP2-N vector

cat.# FP192

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagGFP2-N FP192 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagGFP2 Reporter codon usage mammalian Promoter for TagGFP2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use TagGFP2 expression in mammalian cells; generation of fusions to the TagGFP2 N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TagGFP2
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1393-1395
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1548-1553 & 1577-1582
mRNA 3' ends: 1586 & 1598
f1 single-strand DNA origin: 1645-2100
Bacterial promoter for expression of Kan^r gene
-35 region: 2162-2167
-10 region: 2185-2190
Transcription start point: 2197
SV40 origin of replication: 2441-2576
SV40 early promoter
Enhancer (72-bp tandem repeats): 2274-2345 & 2346-2417
21-bp repeats: 2421-2441, 2442-2462 & 2464-2484
Early promoter element: 2497-2503
Major transcription start points: 2493, 2531, 2537 & 2542
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2625-2627
Stop codon: 3417-3419
G->A mutation to remove Pst I site: 2807
C->A (Arg to Ser) mutation to remove BssH II site: 3153
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3655-3660 & 3668-3673
pUC plasmid replication origin: 4004-4647