pTagBFP-C vector

cat.# FP171

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagBFP-C FP171 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagBFP Reporter codon usage mammalian Promoter for TagBFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use TagBFP expression in mammalian cells; generation of fusions to the TagBFP C-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagBFP
Kozak consensus translation initiation site: 600-610
Start codon (ATG): 607-609
Stop codon: 1384-1386
Last amino acid in TagBFP: 1303-1305
MCS: 1306-1383
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1526-1531 & 1555-1560
mRNA 3' ends: 1564 & 1576
f1 single-strand DNA origin: 1623-2078
Bacterial promoter for expression of Kan^r gene
-35 region: 2140-2145
-10 region: 2163-2168
Transcription start point: 2175
SV40 origin of replication: 2419-2554
SV40 early promoter
Enhancer (72-bp tandem repeats): 2252-2323 & 2324-2395
21-bp repeats: 2399-2419, 2420-2440 & 2442-2462
Early promoter element: 2475-2481
Major transcription start points: 2471, 2509, 2515 & 2520
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2603-2605
Stop codon: 3395-3397
G->A mutation to remove Pst I site: 2785
C->A (Arg to Ser) mutation to remove BssH II site: 3131
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3633-3638 & 3646-3651
pUC plasmid replication origin: 3982-4625