pKatushka2S-B vector

cat.# FP763

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pKatushka2S-B FP763 20 μg € 480 / 240* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type bacterial expression vector Reporter Katushka2S Reporter codon usage mammalian Promoter for Katushka2S T5 promoter/lac operator Host cells prokaryotic Selection ampicillin Replication ColE1 ori Use Source of the Katushka2S coding sequence; Katushka2S expression in bacterial cells

5'-Region

					Age I			Katushka2S
			BamH I				Nco I*
RBS	ATG.	AGA.GGA.TCG.	GGA.TCC.	TT	A.CCG.GT	C.GCC.A	CC.	ATG.G	...

Vector description

pKatushka2S-B is a prokaryotic expression vector encoding far-red fluorescent protein Katushka2S (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of Katushka2S coding sequence. Flanking restriction sites are convenient for excision of Katushka2S sequence and its further insertion into other expression vectors of choice. Alternatively, Katushka2S coding sequence can be amplified by PCR.

The vector can be also used for Katushka2S expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.

Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
Katushka2S coding sequence: 147-854
Lambda t0 transcriptional termination region: 884-978
rrnB T1 transcriptional termination region: 1740-1838
ColE1 origin of replication: 2314
beta-lactamase coding sequence: 3932-3072