pHyPer-nuc vector

cat.# FP944

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pHyPer-nuc FP944 20 μg € 600 / 300* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. The product is not available in certain countries. Please contact Evrogen or your local distributor before ordering. Vector type mammalian expression vector Reporter HyPer Reporter codon usage mammalian / E. coli Promoter for HyPer PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Expression of nuclear-targeted fluorescent hydrogen peroxide sensor HyPer in mammalian cells under the control of CMV promoter; source of nuclear-targeted HyPer coding sequence

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
HyPer-nuc fusion
Start codon (ATG): 603-605
HyPer coding sequence: 603-2036
Nuclear localization signals (NLS): 2058-2130
Stop codon: 2148-2150
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2290-2295 & 2319-2324
mRNA 3' ends: 2328 & 2340
f1 single-strand DNA origin: 2387-2842
Bacterial promoter for expression of Kan^r gene
-35 region: 2904-2909
-10 region: 2927-2932
Transcription start point: 2939
SV40 origin of replication: 3183-3318
SV40 early promoter
Enhancer (72-bp tandem repeats): 3016-3087 & 3088-3159
21-bp repeats: 3163-3183, 3184-3204 & 3206-3226
Early promoter element: 3239-3245
Major transcription start points: 3235, 3273, 3279 & 3284
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3367-3369
Stop codon: 4159-4161
G->A mutation to remove Pst I site: 3549
C->A (Arg to Ser) mutation to remove BssH II site: 3895
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4397-4402 & 4410-4415
pUC plasmid replication origin: 4746-5389