pHyPer-dMito vector

cat.# FP942

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pHyPer-dMito FP942 20 μg € 600 / 300* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. The product is not available in certain countries. Please contact Evrogen or your local distributor before ordering. Vector type mammalian expression vector Reporter HyPer Reporter codon usage mammalian / E. coli Promoter for HyPer PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Expression of mitochondria-targeted fluorescent hydrogen peroxide sensor HyPer in mammalian cells under the control of CMV promoter; source of mitochondria-targeted HyPer coding sequence

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
HyPer-dMito fusion
Start codon (ATG): 597-599
Mitochondrial localization signal 1 (MLS-1): 597-689
Mitochondrial localization signal 2 (MLS-2): 690-782
Start of HyPer coding sequence: 798-800
Stop codon: 2229-2231
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2385-2390 & 2414-2419
mRNA 3' ends: 2423 & 2435
f1 single-strand DNA origin: 2482-2937
Bacterial promoter for expression of Kan^r gene
-35 region: 2999-3004
-10 region: 3022-3027
Transcription start point: 3034
SV40 origin of replication: 3278-3413
SV40 early promoter
Enhancer (72-bp tandem repeats): 3111-3182 & 3183-3254
21-bp repeats: 3258-3278, 3279-3299 & 3301-3321
Early promoter element: 3334-3340
Major transcription start points: 3330, 3368, 3374 & 3379
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3462-3464
Stop codon: 4254-4256
G->A mutation to remove Pst I site: 3644
C->A (Arg to Ser) mutation to remove BssH II site: 3990
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4492-4497 & 4505-4510
pUC plasmid replication origin: 4841-5484