pFusionRed-talin vector

cat.# FP432

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pFusionRed-talin FP432 20 μg € 480 / 240* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter FusionRed Reporter codon usage mammalian Promoter for FusionRed PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red fluorescent labeling of talin

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Talin-FusionRed fusion: 643-9030
Talin: 643-8265
Start codon (ATG): 643-645
Last amino acid in Talin: 8263-8265
FusionRed: 8332-9030
Stop codon: 9028-9030
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 9183-9188 & 9212-9217
mRNA 3' ends: 9221 & 9233
f1 single-strand DNA origin: 9280-9735
Bacterial promoter for expression of Kan^r gene
-35 region: 9797-9802
-10 region: 9820-9825
Transcription start point: 9832
SV40 origin of replication: 10076-10211
SV40 early promoter
Enhancer (72-bp tandem repeats): 9909-9980 & 9981-10052
21-bp repeats: 10056-10076, 10077-10097 & 10099-10119
Early promoter element: 10132-10138
Major transcription start points: 10128, 10166, 10172 & 10177
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 10260-10262
Stop codon: 11052-11054
G->A mutation to remove Pst I site: 10442
C->A (Arg to Ser) mutation to remove BssH II site: 10788
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 11290-11295 & 11303-11308
pUC plasmid replication origin: 11639-12282