pFusionRed-f-mem vector

cat.# FP418

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pFusionRed-f-mem FP418 20 μg € 480 / 240* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter FusionRed Reporter codon usage mammalian Promoter for FusionRed PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red fluorescent labeling of plasma membrane

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
FusionRed
Start codon (ATG): 613-615
Last amino acid in FusionRed: 1306-1308
Farnesylation signal: 1336-1398
Stop codon: 1396-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1590-1595 & 1306-1624
mRNA 3' ends: 1628 & 1640
f1 single-strand DNA origin: 1687-2142
Bacterial promoter for expression of Kan^r gene
-35 region: 2204-2209
-10 region: 2227-2232
Transcription start point: 2239
SV40 origin of replication: 2483-2618
SV40 early promoter
Enhancer (72-bp tandem repeats): 2316-2387 & 2388-2459
21-bp repeats: 2463-2483, 2484-2504 & 2506-2526
Early promoter element: 2539-2545
Major transcription start points: 2535, 2573, 2579 & 2584
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2667-2669
Stop codon: 3459-3461
G->A mutation to remove Pst I site: 2849
C->A (Arg to Ser) mutation to remove BssH II site: 3195
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3697-3702 & 3710-3715
pUC plasmid replication origin: 4046-4689