pFusionRed-endo vector

cat.# FP427

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pFusionRed-endo FP427 20 μg € 480 / 240* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter FusionRed Reporter codon usage mammalian Promoter for FusionRed PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red fluorescent labeling of vesicles of the endocytic pathway

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
FusionRed-Human RhoB GTPase fusion
Start codon (ATG): 613-615
Start of FusionRed coding sequence (ATG): 613-615
Last amino acid in FusionRed: 1306-1308
c-Myc epitope: 1363-1395
Human RhoB GTPase: 1399-1977
Stop codon: 1975-1977
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2169-2174 & 2198-2203
mRNA 3' ends: 2207 & 2219
f1 single-strand DNA origin: 2266-2721
Bacterial promoter for expression of Kan^r gene
-35 region: 2783-2788
-10 region: 2806-2811
Transcription start point: 2818
SV40 origin of replication: 3062-3197
SV40 early promoter
Enhancer (72-bp tandem repeats): 2895-2966 & 2967-3038
21-bp repeats: 3042-3062, 3063-3083 & 3085-3105
Early promoter element: 3118-3124
Major transcription start points: 3114, 3152, 3158 & 3163
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3246-3248
Stop codon: 4038-4040
G->A mutation to remove Pst I site: 3428
C->A (Arg to Ser) mutation to remove BssH II site: 3774
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4276-4281 & 4289-4294
pUC plasmid replication origin: 4625-5268