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pFusionRed-Rab5a vector

cat.# FP431

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pFusionRed-Rab5aFP43120 μg€ 480 / 240*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for FusionRedPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use red fluorescent labeling of Rab-5A protein

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Last amino acid in FusionRed: 1306-1308
Ras-related protein Rab-5A: 1342-1989
Stop codon: 1987-1989
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2150-2155 & 2179-2184
mRNA 3' ends: 2188 & 2200
f1 single-strand DNA origin: 2247-2702
Bacterial promoter for expression of Kanr gene
-35 region: 2764-2769
-10 region: 2787-2792
Transcription start point: 2799
SV40 origin of replication: 3043-3178
SV40 early promoter
Enhancer (72-bp tandem repeats): 2876-2947 & 2948-3019
21-bp repeats: 3023-3043, 3044-3064 & 3066-3086
Early promoter element: 3099-3105
Major transcription start points: 3095, 3133, 3139 & 3144
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3227-3229
Stop codon: 4019-4021
G->A mutation to remove Pst I site: 3409
C->A (Arg to Ser) mutation to remove BssH II site: 3755
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4257-4262 & 4270-4275
pUC plasmid replication origin: 4606-5249

Notice to Purchaser:

FusionRed-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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