pFusionRed-PDHA1 vector

cat.# FP430

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pFusionRed-PDHA1 FP430 20 μg € 480 / 240* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter FusionRed Reporter codon usage mammalian Promoter for FusionRed PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red fluorescent labeling of PDHA1

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
PDHA1-FusionRed fusion: 606-2504
PDHA1: 606-1772
Start codon (ATG): 606-608
Last amino acid in PDHA1: 1770-1772
FusionRed: 1806-2504
Stop codon: 2502-2504
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2657-2662 & 2686-2691
mRNA 3' ends: 2695 & 2707
f1 single-strand DNA origin: 2754-3209
Bacterial promoter for expression of Kan^r gene
-35 region: 3271-3276
-10 region: 3294-3299
Transcription start point: 3306
SV40 origin of replication: 3550-3685
SV40 early promoter
Enhancer (72-bp tandem repeats): 3383-3454 & 3455-3526
21-bp repeats: 3530-3550, 3551-3571 & 3573-3593
Early promoter element: 3606-3612
Major transcription start points: 3602, 3640, 3646 & 3651
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3734-3736
Stop codon: 4526-4528
G->A mutation to remove Pst I site: 3916
C->A (Arg to Ser) mutation to remove BssH II site: 4262
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4764-4769 & 4777-4782
pUC plasmid replication origin: 5113-5756