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pFusionRed-N vector

cat.# FP412

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pFusionRed-NFP41220 μg€ 480 / 240*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for FusionRedPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use FusionRed expression in mammalian cells; generation of fusions to the FusionRed N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1375-1377
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1530-1535 & 1559-1564
mRNA 3' ends: 1568 & 1580
f1 single-strand DNA origin: 1627-2082
Bacterial promoter for expression of Kanr gene
-35 region: 2144-2149
-10 region: 2167-2172
Transcription start point: 2179
SV40 origin of replication: 2423-2558
SV40 early promoter
Enhancer (72-bp tandem repeats): 2256-2327 & 2328-2399
21-bp repeats: 2403-2423, 2424-2444 & 2446-2466
Early promoter element: 2479-2485
Major transcription start points: 2475, 2513, 2519 & 2524
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2607-2609
Stop codon: 3399-3401
G->A mutation to remove Pst I site: 2789
C->A (Arg to Ser) mutation to remove BssH II site: 3135
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3637-3642 & 3650-3655
pUC plasmid replication origin: 3986-4629

Notice to Purchaser:

FusionRed-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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