pFusionRed-H2B vector

cat.# FP421

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pFusionRed-H2B FP421 20 μg € 480 / 240* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter FusionRed Reporter codon usage mammalian Promoter for FusionRed PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red fluorescent labeling of histone H2B

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Histone H2B: 657-1034
FusionRed: 1053-1748
Stop codon: 1749-1751
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1904-1909 & 1933-1938
mRNA 3' ends: 1942 & 1954
f1 single-strand DNA origin: 2001-2456
Bacterial promoter for expression of Kan^r gene
-35 region: 2518-2523
-10 region: 2541-2546
Transcription start point: 2553
SV40 origin of replication: 2797-2932
SV40 early promoter
Enhancer (72-bp tandem repeats): 2630-2701 & 2702-2773
21-bp repeats: 2777-2797, 2798-2818 & 2820-2840
Early promoter element: 2853-2859
Major transcription start points: 2849, 2887, 2893 & 2898
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2981-2983
Stop codon: 3773-3775
G->A mutation to remove Pst I site: 3163
C->A (Arg to Ser) mutation to remove BssH II site: 3509
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4011-4016 & 4024-4029
pUC plasmid replication origin: 4360-5003