pFusionRed-Cx43 vector

cat.# FP417

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pFusionRed-Cx43 FP417 20 μg € 480 / 240* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter FusionRed Reporter codon usage mammalian Promoter for FusionRed PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red fluorescent labeling of connexin 43

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Connexin 43-FusionRed fusion: 824-2689
Start codon: 824-826
Connexin 43: 824-1969
FusionRed: 1991-2689
Stop codon: 2687-2689
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2842-2847 & 2871-2876
mRNA 3' ends: 2880 & 2892
f1 single-strand DNA origin: 2939-3394
Bacterial promoter for expression of Kan^r gene
-35 region: 3456-3461
-10 region: 3479-3484
Transcription start point: 3491
SV40 origin of replication: 3735-3870
SV40 early promoter
Enhancer (72-bp tandem repeats): 3568-3639 & 3640-3711
21-bp repeats: 3715-3735, 3736-3756 & 3758-3778
Early promoter element: 3791-3797
Major transcription start points: 3787, 3825, 3831 & 3836
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3919-3921
Stop codon: 4711-4713
G->A mutation to remove Pst I site: 4101
C->A (Arg to Ser) mutation to remove BssH II site: 4447
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4949-4954 & 4962-4967
pUC plasmid replication origin: 5298-5941