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pFusionRed-Cx43 vector

cat.# FP417

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pFusionRed-Cx43FP41720 μg€ 480 / 240*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for FusionRedPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use red fluorescent labeling of connexin 43

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Connexin 43-FusionRed fusion: 824-2689
Start codon: 824-826
Connexin 43: 824-1969
FusionRed: 1991-2689
Stop codon: 2687-2689
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2842-2847 & 2871-2876
mRNA 3' ends: 2880 & 2892
f1 single-strand DNA origin: 2939-3394
Bacterial promoter for expression of Kanr gene
-35 region: 3456-3461
-10 region: 3479-3484
Transcription start point: 3491
SV40 origin of replication: 3735-3870
SV40 early promoter
Enhancer (72-bp tandem repeats): 3568-3639 & 3640-3711
21-bp repeats: 3715-3735, 3736-3756 & 3758-3778
Early promoter element: 3791-3797
Major transcription start points: 3787, 3825, 3831 & 3836
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3919-3921
Stop codon: 4711-4713
G->A mutation to remove Pst I site: 4101
C->A (Arg to Ser) mutation to remove BssH II site: 4447
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4949-4954 & 4962-4967
pUC plasmid replication origin: 5298-5941

Notice to Purchaser:

FusionRed-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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