TurboYFP normalized excitation (thin line) and emission (thick line) spectra.
Spectra viewer tool Download TurboYFP spectra (xls)
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| | Molecular weight, kDa | 26 | | Polypeptide length, aa | 234 | | Fluorescence color | yellow | | Excitation maximum, nm | 525 | | Emission maximum, nm | 538 | | Quantum yield | 0.53 | | Extinction coefficient, M-1cm-1 | 105 000 | | Brightness* | 55.7 | | Brightness, % of EGFP | 169 | | pKa | 5.9 | | Structure | dimer | | Aggregation | at high concentrations | | Maturation rate at 37°C | super fast | | Photostability | high | | Cell toxicity | at high concentrations | | Main advantages | superbright and fast-maturing yellow fluorescent protein | | Possible limitations | dimer, limited applicability for fusions generation; can form aggregates at high concentration |
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