Near-infrared fluorescent protein TurboFP650

- Bright near-infrared fluorescence
- Fast maturation, high photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for whole body imaging and multicolor applications

Main properties

TurboFP650 normalized excitation (thin line) and emission (thick line) spectra. Spectra viewer tool Download TurboFP650 spectra (xls)

CHARACTERISTIC * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. ** Brightness in infrared is a product of extinction coefficient at 635 nm, quantum yield and emission fraction between 700 nm and 900 nm, divided by 1000. Molecular weight, kDa 26 Polypeptide length, aa 234 Fluorescence color near-infrared Excitation maximum, nm 592 Emission maximum, nm 650 Quantum yield 0.24 Extinction coefficient, M-1cm-1 65 000 Brightness* 15.6 Brightness, % of EGFP 47 Extinction coefficient, M-1cm-1 at 635 nm 4 300 Quantum yield in infrared (700-900 nm) 0.07 Brightness in infrared** 0.3 pKa 5.7 Structure dimer Aggregation no Maturation rate at 37°C super fast Photostability high Cell toxicity not observed Main advantages the brightest fluorescent protein with emission maxima above 635 nm; fast maturation; high pH-stability and photostability Possible limitations dimer, limited applicability for fusions generation