SLIM
GFP with short fluorescence lifetime

- Green fluorescence
- Short fluorescence lifetime (1300 ps, in vitro)
- Recommended for fluorescence lifetime imaging microscopy (FLIM)
- Fast maturation and high photostability

Performance and use

SLIM can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with SLIM expression vectors produce bright fluorescence in 16-18 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed.

It can be used in multiparameter FLIM in green channel.

	Color-coded fluorescence lifetime imaging microscopy (FLIM) image of live HeLa cells co-expressing BrUSLEE (mitochondria), SLIM (nuclei) and TagGFP2 (cytoplasm). Color hue indicates mean lifetime at each pixel (red-orange – 750-900 ps, green – 1100-1400 ps, cyan-blue – 2700-3100 ps). Single-photon fluorescence excitation at 488 nm was used to acquire these images.