SLIM
GFP with short fluorescence lifetime

- Green fluorescence
- Short fluorescence lifetime (1300 ps, in vitro)
- Recommended for fluorescence lifetime imaging microscopy (FLIM)
- Fast maturation and high photostability

Main properties

SLIM normalized excitation (thin line) and emission (thick line) spectra. Download SLIM spectra (xls)

CHARACTERISTIC * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Molecular weight, kDa 27 Polypeptide length, aa 239 Fluorescence color green Excitation maximum, nm 488 Emission maximum, nm 508 Quantum yield 0.06 Extinction coefficient, M-1cm-1 70 000 Brightness* 4.2 Brightness, % of EGFP 13 Fluorescence lifetime, ps 1300 Fluorescence intensity decay nearly single-exponential Aggregation no Maturation rate at 37°C fast Photostability high Cell toxicity not observed Main advantages green fluorescent protein with shortened fluorescence lifetime