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    PA-TagRFP

Photoactivatable red fluorescent protein PA-TagRFP

- Monomer, successful performance in fusions
- Non-fluorescent before photoactivation
- Irreversible photoactivation to a red fluorescent form by UV-violet light irradiation
- High brightness and photostability
- Recommended for super-resolution imaging


Available variants and fusions
VariantDescriptionRelated vectorCat.#
Humanized PA-TagRFP PA-TagRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. pPA-TagRFP-C FP811
pPA-TagRFP-N FP812
PA-TagRFP-tubulin fusion Human α-tubulin is fused to the PA-TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-tubulin in living cells. pPA-TagRFP-tubulin FP814
PA-TagRFP-H2B fusion Human histone H2B is fused to the PA-TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of histone H2B in living cells. pPA-TagRFP-H2B FP815


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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