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Photoactivatable red fluorescent protein PA-TagRFP
- Monomer, successful performance in fusions
- Non-fluorescent before photoactivation
- Irreversible photoactivation to a red fluorescent form by UV-violet light irradiation
- High brightness and photostability
- Recommended for super-resolution imaging
Available variants and fusions
Variant | Description | Related vector | Cat.# |
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Humanized PA-TagRFP
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PA-TagRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
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pPA-TagRFP-C
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FP811
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pPA-TagRFP-N
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FP812
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PA-TagRFP-tubulin fusion
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Human α-tubulin is fused to the PA-TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-tubulin in living cells.
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pPA-TagRFP-tubulin
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FP814
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PA-TagRFP-H2B fusion
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Human histone H2B is fused to the PA-TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of histone H2B in living cells.
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pPA-TagRFP-H2B
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FP815
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References:
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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