Photoactivatable red fluorescent protein PA-TagRFP

- Monomer, successful performance in fusions
- Non-fluorescent before photoactivation
- Irreversible photoactivation to a red fluorescent form by UV-violet light irradiation
- High brightness and photostability
- Recommended for super-resolution imaging

Main properties

Normalized excitation (thin line) and emission (thick line) spectra for activated PA-TagRFP. Download PA-TagRFP spectra (xls)

CHARACTERISTIC before  /  after photoactivation * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Fluorescence color NO   /   red Excitation maximum, nm -   /   562 Emission maximum, nm -   /   595 Quantum yield nd   /   0.38 Extinction coefficient, M-1cm-1 nd   /   66 000 Brightness* 0   /   25.1 pKa nd   /   5.3 Activating light UV-violet (e.g. 390-420 nm) Calculated contrast, fold ~540 Structure monomer Cell toxicity not observed Aggregation no Maturation rate at 37°C fast Molecular weight, kDa 27 Polypeptide length, aa 237