Red fluorescent protein JRed

- True-red fluorescence
- Proven suitability to create stably transfected cell lines

Performance and use

JRed can be expressed in eukaryotic cells; however, it is not appropriate for expression in prokaryotic.

JRed possesses relatively fast photobleaching rate upon arc lamp irradiation. At the same time, it exhibits high photostability when excited by 543 nm laser line in a confocal microscope, with the photobleaching time several times longer compared with DsRed2. JRed could show phototoxicity when bleached.

Mammalian cells transiently transfected with JRed vector give red fluorescence without visible aggregation. Mammalian cells transiently transfected with JRed expression vectors produce bright fluorescence in 24 hours after transfection.

JRed suitability to generate stably transfected cells has been proven by Marinpharm company.

Fluorescent microscopy of stably transfected mammalian cells expressing JRed in cytosol. Images were kindly provided by Dr. Christian Petzelt (Marinpharm).

Despite dimerization capacity, JRed demonstrates successful performance in fusions with subcellular localization signals and many cellular proteins including BH3 interacting domain death agonist (BID), nucleolar protein fibrillarin, dopamin transporter (hDAT). However, we recommend that you use monomeric TagFPs for protein labeling applications.

Fluorescent microscopy of mammalian cells expressing JRed fusions. Image of stably transfected PtK rat kangaroo cells expressing mitochondria-targeted JRed was kindly provided by Dr. Christian Petzelt (Marinpharm).