Red fluorescent protein JRed

- True-red fluorescence
- Proven suitability to create stably transfected cell lines

Main properties

JRed normalized excitation (thin line) and emission (thick line) spectra. Download JRed spectra (xls)

CHARACTERISTIC * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Molecular weight, kDa 27 Polypeptide length, aa 242 Fluorescence color true-red Excitation maximum, nm 584 Emission maximum, nm 610 Quantum yield 0.20 Extinction coefficient, M-1cm-1 44 000 Brightness* 8.8 Brightness, % of EGFP 26 pKa 5.0 Structure weak dimer Aggregation no Maturation rate at 37°C slow Photostability medium Cell toxicity at certain excitation wavelengths Main advantages true-red fluorescent protein with good compatibility with popular filter sets Possible limitations dimer, limited applicability for fusions generation; unsuitable for expression in procaryotes