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GFP with subnanosecond fluorescence lifetime

- Bright green fluorescence
- Fast maturation and enhanced photostability
- Short fluorescence lifetime (800 ps, in vitro)
- Recommended for fluorescence lifetime imaging microscopy (FLIM)

Performance and use

BrUSLEE can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with BrUSLEE expression vectors produce bright fluorescence in 16-18 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed.

It can be used in multiparameter FLIM in green channel.

Color-coded fluorescence lifetime imaging microscopy (FLIM) image of live HeLa cells co-expressing BrUSLEE (mitochondria), SLIM (nuclei) and TagGFP2 (cytoplasm).

Color hue indicates mean lifetime at each pixel (red-orange – 750-900 ps, green – 1100-1400 ps, cyan-blue – 2700-3100 ps). Single-photon fluorescence excitation at 488 nm was used to acquire these images.

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