BrUSLEE can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with BrUSLEE expression vectors produce bright fluorescence in 16-18 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed.
 | Color-coded fluorescence lifetime imaging microscopy (FLIM) image of live HeLa cells co-expressing BrUSLEE (mitochondria), SLIM (nuclei) and TagGFP2 (cytoplasm).
Color hue indicates mean lifetime at each pixel (red-orange – 750-900 ps, green – 1100-1400 ps, cyan-blue – 2700-3100 ps). Single-photon fluorescence excitation at 488 nm was used to acquire these images.
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