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GFP with subnanosecond fluorescence lifetime

- Bright green fluorescence
- Fast maturation and enhanced photostability
- Short fluorescence lifetime (800 ps, in vitro)
- Recommended for fluorescence lifetime imaging microscopy (FLIM)

Main properties

BrUSLEE spectra

BrUSLEE normalized excitation (thin line) and emission (thick line) spectra.

Download BrUSLEE spectra (xls)

* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa27
Polypeptide length, aa239
Fluorescence colorgreen
Excitation maximum, nm487
Emission maximum, nm509
Quantum yield0.3
Extinction coefficient, M-1cm-186 000
Brightness, % of EGFP78
Fluorescence lifetime, ps800
Fluorescence intensity decaynearly single-exponential
Maturation rate at 37°Cfast
Cell toxicitynot observed
Main advantagesbright green fluorescent protein with subnanosecond fluorescence lifetime
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