BrUSLEE
GFP with subnanosecond fluorescence lifetime

- Bright green fluorescence
- Fast maturation and enhanced photostability
- Short fluorescence lifetime (800 ps, in vitro)
- Recommended for fluorescence lifetime imaging microscopy (FLIM)

Main properties

BrUSLEE normalized excitation (thin line) and emission (thick line) spectra. Download BrUSLEE spectra (xls)

CHARACTERISTIC * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Molecular weight, kDa 27 Polypeptide length, aa 239 Fluorescence color green Excitation maximum, nm 487 Emission maximum, nm 509 Quantum yield 0.3 Extinction coefficient, M-1cm-1 86 000 Brightness* 26 Brightness, % of EGFP 78 Fluorescence lifetime, ps 800 Fluorescence intensity decay nearly single-exponential Aggregation no Maturation rate at 37°C fast Photostability high Cell toxicity not observed Main advantages bright green fluorescent protein with subnanosecond fluorescence lifetime