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    pTagBFP-N

pTagBFP-N vector

cat.# FP172

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pTagBFP-NFP17220 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Vector typemammalian expression vector
ReporterTagBFP
Reporter codon usagemammalian
Promoter for TagBFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use TagBFP expression in mammalian cells; generation of fusions to the TagBFP N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TagBFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1378-1380
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1534-1539 & 1563-1568
mRNA 3' ends: 1572 & 1584
f1 single-strand DNA origin: 1631-2086
Bacterial promoter for expression of Kanr gene
-35 region: 2148-2153
-10 region: 2171-2176
Transcription start point: 2183
SV40 origin of replication: 2427-2562
SV40 early promoter
Enhancer (72-bp tandem repeats): 2260-2331 & 2332-2403
21-bp repeats: 2407-2427, 2428-2448 & 2450-2470
Early promoter element: 2483-2489
Major transcription start points: 2479, 2517, 2523 & 2528
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2611-2613
Stop codon: 3403-3405
G->A mutation to remove Pst I site: 2793
C->A (Arg to Ser) mutation to remove BssH II site: 3139
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3641-3646 & 3654-3659
pUC plasmid replication origin: 3990-4633


Notice to Purchaser:

TagBFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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