TurboFP650

Near-infrared fluorescent protein TurboFP650

- Bright near-infrared fluorescence
- Fast maturation, high photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for whole body imaging and multicolor applications

Main properties

TurboFP650 spectra

TurboFP650 normalized excitation (thin line) and emission (thick line) spectra.

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Download TurboFP650 spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
** Brightness in infrared is a product of extinction coefficient at 635 nm, quantum yield and emission fraction between 700 nm and 900 nm, divided by 1000.
Molecular weight, kDa26
Polypeptide length, aa234
Fluorescence colornear-infrared
Excitation maximum, nm592
Emission maximum, nm650
Quantum yield0.24
Extinction coefficient, M-1cm-165 000
Brightness*15.6
Brightness, % of EGFP47
Extinction coefficient, M-1cm-1 at 635 nm4 300
Quantum yield in infrared (700-900 nm)0.07
Brightness in infrared**0.3
pKa5.7
Structuredimer
Aggregationno
Maturation rate at 37°Csuper fast
Photostabilityhigh
Cell toxicitynot observed
Main advantagesthe brightest fluorescent protein with emission maxima above 635 nm; fast maturation; high pH-stability and photostability
Possible limitationsdimer, limited applicability for fusions generation
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