PA-TagRFP

Photoactivatable red fluorescent protein PA-TagRFP

- Monomer, successful performance in fusions
- Non-fluorescent before photoactivation
- Irreversible photoactivation to a red fluorescent form by UV-violet light irradiation
- High brightness and photostability
- Recommended for super-resolution imaging

Main properties

PA-TagRFP spectra

Normalized excitation (thin line) and emission (thick line) spectra for activated PA-TagRFP.

Download PA-TagRFP spectra (xls)

CHARACTERISTICbefore  /  after photoactivation
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Fluorescence colorNO   /   red
Excitation maximum, nm-   /   562
Emission maximum, nm-   /   595
Quantum yieldnd   /   0.38
Extinction coefficient, M-1cm-1nd   /   66 000
Brightness*0   /   25.1
pKand   /   5.3
Activating lightUV-violet (e.g. 390-420 nm)
Calculated contrast, fold~540
Structuremonomer
Cell toxicitynot observed
Aggregationno
Maturation rate at 37°Cfast
Molecular weight, kDa27
Polypeptide length, aa237
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