Photoactivatable red fluorescent protein PA-TagRFP
- Monomer, successful performance in fusions
- Non-fluorescent before photoactivation
- Irreversible photoactivation to a red fluorescent form by UV-violet light irradiation
- High brightness and photostability
- Recommended for super-resolution imaging
Recommended antibodies, filter sets and laser lines
PA-TagRFP can be recognized using Anti-tRFP antibody (Cat.# AB233-AB234) available from Evrogen.
PA-TagRFP is non-fluorescent before light activation. Upon UV-violet irradiation the protein irreversibly converts to its red fluorescent form.
PA-TagRFP can be activated during both widefield imaging (e.g. the Arc-lamp irradiation, 100xoil objective, 390-420 nm, 10-50 mW/cm2) and confocal laser scanning imaging (e.g. 405 nm laser line, estimated < 2.5 W/cm2 at the sample). Maximal efficiency of photoactivation for PA-TagRFP is observed at 390-420 nm. The photoactivation efficiency drops dramatically with the wavelength increasing above 420 nm.
The source of irradiation, irradiation time and intensity of activating UV-violet light must be individually adjusted for particular instrumentation and intended application.
TRITC filter set or similar can be used for visualization of activated PA-TagRFP. Omega Optical filter sets QMAX-Red and XF174 are recommended.