KillerRed

Genetically-encoded photosensitizer KillerRed

- Light-induced production of reactive oxygen species
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required for chromophore maturation
- Not toxic before activation by green or orange light irradiation
- Recommended for selective light-induced protein inactivation and cell killing

Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They can be used for precise inactivation of selected proteins in chromophore-assisted light inactivation (CALI) technique and for the light-induced cell killing, for example in photodynamic therapy. Besides KillerRed protein (and its derivative KillerOrange), all known to date photosensitizers require chemical compounds that should be introduced into living systems exogenously. Red fluorescent protein KillerRed is the first genetically-encoded photosensitizer [Bulina et al., 2006a]. Unlike chemical analogs, KillerRed can be directly expressed by target cells, both individually and in fusion with a target protein. It shows no cell toxic effects before light activation. Upon green or orange light irradiation, KillerRed generates ROS that damage the neighboring molecules.

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.


KillerRed expression/source vectors

pKillerRed-C FP961 Mammalian expression vector encoding humanized KillerRed and allowing its expression and generation of fusions to the KillerRed C-terminus 20 μg € 400 / 200*
pKillerRed-N FP962 Mammalian expression vector encoding humanized KillerRed and allowing its expression and generation of fusions to the KillerRed N-terminus 20 μg € 400 / 200*
pKillerRed-B FP963 Bacterial expression vector; source of the KillerRed coding sequence 20 μg € 400 / 200*
pKillerRed-dMito FP964 Mammalian expression vector encoding mitochondria-targeted KillerRed 20 μg € 400 / 200*
pKillerRed-mem FP966 Mammalian expression vector encoding membrane-targeted KillerRed 20 μg € 400 / 200*


Antibody against KillerRed

Anti-KillerRed
antibody
AB961 Rabbit polyclonal antibody against KillerRed, KillerOrange, ArrestRed, and JRed 100 μg € 150
AB962 200 μg € 200

References:

  • Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA. A genetically encoded photosensitizer. Nat Biotechnol. 2006a; 24 (1):95-9. / pmid: 16369538
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