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    pKillerRed-B

pKillerRed-B vector

cat.# FP963

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pKillerRed-BFP96320 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typebacterial expression vector
ReporterKillerRed
Reporter codon usagemammalian
Promoter for KillerRedT5 promoter/lac operator
Host cellsprokaryotic
Selectionampicillin
ReplicationColE1 ori
Use Source of the KillerRed coding sequence; KillerRed expression in bacterial cells
5'-Region
KillerRed
BamH I
RBS ATG. AGA.GGA.TCG. GGA. TCC. ATG.GGT.TCA.GAG
3'-Region
Stop
Hind III
GGT.AGC.GAC.GAG.GAT. TG A. AAG.CTT

Vector description

pKillerRed-B is a prokaryotic expression vector encoding photosensitizer KillerRed (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of KillerRed coding sequence. Flanking restriction sites are convenient for excision of KillerRed sequence and its further insertion into other expression vectors of choice. Alternatively, KillerRed coding sequence can be amplified by PCR.

Note: The plasmid DNA was isolated from dam+-methylated E. coli. Therefore some restriction sites are blocked by methylation. If you wish to digest the vector using such sites you will need to transform the vector into a dam- host and make fresh DNA.

The vector can be also used for KillerRed expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.


Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
KillerRed coding sequence: 133-852
Lambda t0 transcriptional termination region: 874-968
rrnB T1 transcriptional termination region: 1730-1828
ColE1 origin of replication: 2304
beta-lactamase coding sequence: 3922-3062


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248

Notice to Purchaser:

KillerRed-related materials (also referred to as "Products") are intended for research use only.

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