FusionRed

Red fluorescent protein FusionRed

- Superior performance in fusions
- Low cytotoxicity
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling and long-term experiments


Available variants and fusions
VariantDescriptionRelated vectorCat.#Click for image
Humanized FusionRed FusionRed codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. pFusionRed-C FP411
pFusionRed-N FP412
pFusionRed-B FP438
FusionRed-actin fusion Human β-actin is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of β-actin in living cells. pFusionRed-actin FP413
FusionRed-annexin fusion Human annexin is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of annexin in living cells. pFusionRed-annexin FP414
FusionRed-CD151 fusion Human CD151 is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of CD151 in living cells. pFusionRed-CD151 FP415
FusionRed-Cx26 fusion Rat connexin 26 is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 26 in living cells. pFusionRed-Cx26 FP416
FusionRed-Cx43 fusion Rat connexin 43 is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 43 in living cells. pFusionRed-Cx43 FP417
FusionRed-f-mem fusion 20 amino acid farnesylation signal from c-Ha-Ras is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of plasma membrane. pFusionRed-f-mem FP418
FusionRed-Golgi fusion Golgi targeting sequence (fragment of human β-1,4-galactosyltransferase) is fused to the FusionRed N-terminus. When expressed in mammalian cells, this variant provides red fluorescent labeling of Golgi apparatus. pFusionRed-Golgi FP419
FusionRed-ER fusion Signal sequence of calreticulin is fused to the FusionRed N-terminus and the endoplasmic reticulum retention sequence KDEL is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of the lumen of the endoplasmic reticulum. pFusionRed-ER FP420
FusionRed-H2B fusion Human histone H2B is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of histone H2B in living cells. pFusionRed-H2B FP421
FusionRed-laminB1 fusion Human lamin B1 is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of lamin B1 in living cells. pFusionRed-laminB1 FP422
FusionRed-vimentin fusion Human vimentin is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of vimentin in living cells. pFusionRed-vimentin FP423
FusionRed-vinculin fusion Human vinculin is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of vinculin in living cells. pFusionRed-vinculin FP424
FusionRed-zyxin fusion Human zyxin is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of zyxin in living cells. pFusionRed-zyxin FP425
FusionRed-actinin fusion Human α-actinin is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-actinin in living cells. pFusionRed-actinin FP426
FusionRed-endo fusion Human RhoB GTPase is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of vesicles of the endocytic pathway. pFusionRed-endo FP427
FusionRed-MAP4 fusion Microtubule binding domain of mouse microtubule-associated protein 4 (MAP4) is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of microtubules in living cells. pFusionRed-MAP4 FP428
FusionRed-paxillin fusion Chicken paxillin is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of paxillin in living cells. pFusionRed-paxillin FP429
FusionRed-PDHA1 fusion Human pyruvate dehydrogenase alpha 1 (PDHA1) is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of PDHA1 in living cells. pFusionRed-PDHA1 FP430
FusionRed-Rab5a fusion Human Ras-related protein Rab-5A is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of Rab-5A in living cells. pFusionRed-Rab5a FP431
FusionRed-talin fusion Mouse talin-1 protein is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of talin in living cells. pFusionRed-talin FP432
FusionRed-tubulin fusion Human α-tubulin is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-tubulin in living cells. pFusionRed-tubulin FP433
FusionRed-VE-cadherin fusion Human VE-cadherin is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of VE-cadherin in living cells. pFusionRed-cadherin FP434
FusionRed-CDC42 fusion CDC42 is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of CDC42 in living cells. pFusionRed-CDC42 FP435
FusionRed-myosin fusion Myosin regulatory light polypeptide 9 is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of myosin in living cells. pFusionRed-myosin FP436
FusionRed-PCNA fusion Proliferating cell nuclear antigen (PCNA) is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of proliferating cell nuclear antigen (PCNA) in living cells. pFusionRed-PCNA FP437


References:

  • Fliegel L, Burns K, MacLennan DH, Reithmeier RA, Michalak M. Molecular cloning of the high affinity calcium-binding protein (calreticulin) of skeletal muscle sarcoplasmic reticulum. J Biol Chem. 1989; 264 (36):21522-8. / pmid: 2600080
  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
  • Munro S, Pelham HR. A C-terminal signal prevents secretion of luminal ER proteins. Cell. 1987; 48 (5):899-907. / pmid: 3545499
  • Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM, Shcherbo D. A monomeric red fluorescent protein with low cytotoxicity. Nat Commun. 2012; 3 :1204. doi: 10.1038/ncomms2208 / pmid: 23149748
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