peTurboGFP-PRL-dest1 vector

cat.# FP523

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price peTurboGFP-PRL-dest1 FP523 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type promoterless expression vector Reporter TurboGFP Reporter codon usage mammalian Promoter for TurboGFP NO Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Monitoring of activity of different promoters and promoter/enhancer combinations

Location of features

MCS: 12-89
TurboGFP-dest1
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Last amino acid in TurboGFP: 790-792
Amino acid residues of mouse ornithine decarboxylase (MODC) PEST sequence: 808-930
Stop codon: 928-930
Fragment of human beta globin (HBB) gene
Last 35 bp of HBB exon 2: 939-973
HBB intron 2: 974-1823
First 233 bp of HBB exon 3: 1824-2057
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2199-2204 & 2228-2233
mRNA 3' ends: 2237 & 2249
f1 single-strand DNA origin: 2296-2751
Bacterial promoter for expression of Kan^r gene
-35 region: 2813-2818
-10 region: 2836-2841
Transcription start point: 2848
SV40 origin of replication: 3092-3227
SV40 early promoter
Enhancer (72-bp tandem repeats): 2925-2996 & 2997-3068
21-bp repeats: 3072-3092, 3093-3113 & 3115-3135
Early promoter element: 3148-3154
Major transcription start points: 3144, 3182, 3188 & 3193
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3276-3278
Stop codon: 4068-4070
G->A mutation to remove Pst I site: 3458
C->A (Arg to Ser) mutation to remove BssH II site: 3804
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4306-4311 & 4319-4324
pUC plasmid replication origin: 4655-5298