peNirFP-N vector

cat.# FP743

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price peNirFP-N FP743 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter NirFP Reporter codon usage mammalian Promoter for NirFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use NirFP expression in mammalian cells; generation of fusions to the NirFP N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
NirFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1381-1383
Fragment of human beta globin (HBB) gene: 1392-2510
Last 35 bp of HBB exon 2: 1392-1426
HBB intron 2: 1427-2277
First 233 bp of HBB exon3: 2278-2510
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2652-2657 & 2681-2686
mRNA 3' ends: 2690 & 2702
f1 single-strand DNA origin: 2749-3204
Bacterial promoter for expression of Kan^r gene
-35 region: 3266-3271
-10 region: 3289-3294
Transcription start point: 3301
SV40 origin of replication: 3545-3680
SV40 early promoter
Enhancer (72-bp tandem repeats): 3378-3449 & 3450-3521
21-bp repeats: 3525-3545, 3546-3566 & 3568-3588
Early promoter element: 3601-3607
Major transcription start points: 3597, 3635, 3641 & 3646
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3729-3731
Stop codon: 4521-4523
G->A mutation to remove Pst I site: 3911
C->A (Arg to Ser) mutation to remove BssH II site: 4257
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4759-4764 & 4772-4777
pUC plasmid replication origin: 5108-5751